Dieter Laser plays the role of Dr. Heiter, a disturbed surgeon obsessed with creating a human centipede by surgically connecting his victims.
Ashley C. Williams portrays Lindsay, one of the unfortunate individuals kidnapped by Dr. Heiter to be part of his macabre experiment.
Ashlynn Yennie takes on the role of Jenny, another victim who becomes part of Dr. Heiter's grotesque creation.
Akihiro Kitamura plays Katsuro, a man who falls prey to Dr. Heiter's sadistic plan to connect his captives in an unimaginable way.
Andreas Leupold portrays Detective Kranz, one of the investigators on the trail of Dr. Heiter and his horrifying experiments.
Peter Blankenstein takes on the role of Detective Voller, the partner of Detective Kranz in the pursuit of the deranged Dr. Heiter.
Rosemary Annabella provides the voice of Amy, a character in the film whose fate is intertwined with the nightmarish events orchestrated by Dr. Heiter.
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