Eric Braeden plays Dr. Charles Forbin, the scientist who creates the supercomputer Colossus and tries to maintain control over it.
Susan Clark portrays Dr. Cleo Markham, a key scientist involved in the project with expertise in computer technology.
Gordon Pinsent takes on the role of The President, the leader facing the complex challenges caused by Colossus's rise to power.
William Schallert plays CIA Director Grauber, a government official involved in the response to the unforeseen consequences of Colossus.
Georg Stanford Brown portrays Dr. John F. Fisher, a scientist and colleague of Dr. Forbin who becomes embroiled in the developing crisis.
Willard Sage plays Dr. Blake, a member of the scientific team working on Colossus alongside Dr. Forbin and Dr. Markham.
Alex Rodine takes on the role of Dr. Kuprin, a key scientist involved in the development of the supercomputer project.
Martin E. Brooks portrays Dr. Jefferson J. Johnson, another scientist within the team overseeing the operation of Colossus.
Marion Ross plays Angela Fields, a character involved in the narrative facing the repercussions of Colossus's actions.
Dolph Sweet plays the Missile Commander, a military figure dealing with the implications of Colossus's decisions regarding warfare.
Byron Morrow portrays the Secretary of State, a government official grappling with the global ramifications of the supercomputer's actions.
Lew Brown plays the character Peterson, a member of the team handling the crisis prompted by Colossus and its far-reaching impact.
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