Alfre Woodard portrays Eunice Evers, R.N., a dedicated nurse who is part of the Tuskegee Study.
Laurence Fishburne plays the role of Caleb Humphries, one of the subjects in the Tuskegee Study.
Craig Sheffer portrays Dr. Douglas, a doctor involved in the unethical Tuskegee Study.
Joe Morton plays Dr. Sam Brodus, a physician connected to the Tuskegee Study.
Obba Babatundé portrays Willie Johnson, one of the participants in the Tuskegee Study.
Ossie Davis plays the role of Mr. Evers, a character entangled in the events of the Tuskegee Study.
Peter Stelzer portrays a Senator involved in the Tuskegee Study.
Donzaleigh Abernathy plays Nurse Betty, a healthcare provider within the context of the Tuskegee Study.
Robert Benedetti portrays a Senator connected to the events of the Tuskegee Study.
Von Coulter plays the character Hodman Bryan in the movie.
Thom Gossom Jr. portrays Ben Washington in Miss Evers' Boys.
E.G. Marshall plays The Senate Chairman in the film.
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