Derek Jacobi provides the voice for Nicodemus, a wise and mystical character who guides Mrs. Brisby on her journey.
Elizabeth Hartman voices the protagonist, Mrs. Brisby, a courageous mouse determined to protect her family.
Arthur Malet lends his voice to Mr. Ages, an elderly mouse skilled in medicine and science.
Dom DeLuise brings humor to the character of Jeremy, a clumsy and well-meaning crow.
Hermione Baddeley voices Auntie Shrew, a strict and overprotective mouse who looks out for Mrs. Brisby.
Shannen Doherty voices Teresa, one of Mrs. Brisby's children who also gets involved in the adventure.
Wil Wheaton provides the voice for Martin, another one of Mrs. Brisby's children who faces his own challenges.
Jodi Hicks voices Cynthia, the daughter of Mrs. Brisby who has a more timid and delicate nature.
Ina Fried voices Timothy, Mrs. Brisby's youngest child who often requires special attention and care.
John Carradine voices the Great Owl, a mysterious and ancient creature who imparts wisdom.
Peter Strauss lends his voice to Justin, a brave and noble rat who becomes an ally to Mrs. Brisby.
Paul Shenar voices Jenner, a malevolent and power-hungry rat who opposes the other characters.
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