Jeffrey Combs portrays the eccentric and ambitious Herbert West, a medical student obsessed with conquering death through his experiments with reanimation.
Bruce Abbott plays Dan Cain, a talented medical student who becomes entangled in Herbert West's dangerous and unethical experiments.
Barbara Crampton is Megan Halsey, the compassionate daughter of the medical school's dean who becomes involved in the horrifying events unfolding around her.
David Gale portrays the sinister Dr. Carl Hill, a rival of Herbert West with nefarious intentions and a dark secret of his own.
Robert Sampson plays Dean Alan Halsey, Megan's father and the head of the medical school, who becomes a victim of the chaos unleashed by Herbert West.
Carolyn Purdy-Gordon is Dr. Harrod, a fellow medical professional who witnesses the disturbing consequences of Herbert West's experiments.
Peter Kent appears as Melvin the Re-Animated, an unsettling undead creation brought back to life by Herbert West.
Ian Patrick Williams plays the Swiss Professor, a minor character involved in the world of academia where Herbert West and his experiments take place.
Bunny Summers appears as the Swiss Woman Doctor, another minor character connected to the medical field in the film.
Al Berry portrays Dr. Gruber, a brief but memorable character affected by the chaos caused by Herbert West's reanimation experiments.
Barbara Pieters appears as a Nurse, a supporting character in the medical setting where the events of the film unfold.
Annyce Holzman plays the E-R Patient Corpse, an eerie and unsettling figure revived through Herbert West's experiments.
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