Paul Reubens portrays Pee-wee Herman, a man-child with a love for all things quirky and unconventional.
Elizabeth Daily plays Dottie, Pee-wee's caring and supportive friend who helps him in his quest to find his stolen bike.
Mark Holton stars as Francis, Pee-wee's spoiled and resentful acquaintance who plays a pivotal role in the adventure.
Diane Salinger takes on the role of Simone, an enigmatic woman who captures Pee-wee's attention during his escapades.
Judd Omen portrays Mickey, one of the eccentric characters Pee-wee encounters on his journey.
Irving Hellman plays Mr. Crowtrey, a character who adds a touch of mystery to Pee-wee's Big Adventure.
Monte Landis takes on the role of Mario, a colorful character who crosses paths with Pee-wee during his search.
Damon Martin stars as Chip, a young character who plays a minor yet memorable part in Pee-wee's escapades.
David Glasser appears as BMX Kid, adding a dose of excitement to Pee-wee's adventurous journey.
Gregory Brown plays another BMX Kid, contributing to the energetic and youthful spirit of the film.
Daryl Keith Roach stars as Chuck, a character who encounters Pee-wee in unexpected circumstances.
Bill Cable appears as Policeman #1, bringing a touch of authority to the quirky world of Pee-wee's Big Adventure.
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