John Belushi plays John "Bluto" Blutarski, a loud and rebellious fraternity member known for his outrageous behavior.
Tim Matheson portrays Eric "Otter" Stratton, a smooth-talking fraternity brother who often charms his way into trouble.
John Vernon acts as Dean Vernon Wormer, the strict dean of Faber College who opposes the Delta Tau Chi fraternity.
Verna Bloom plays Marion Wormer, the supportive wife of Dean Wormer who often finds herself caught in the conflict.
Tom Hulce portrays Larry "Pinto" Kroger, a naive and eager-to-please member of the Delta fraternity.
Cesare Danova plays Mayor Carmine DePasto, the town mayor who gets involved with the fraternity's antics.
Peter Riegert plays Donald "Boon" Schoenstein, a fraternity brother who is in a romantic relationship with a college girl.
Mary Louise Weller portrays Mandy Pepperidge, a love interest for several characters in the film.
Stephen Furst plays Kent "Flounder" Dorfman, a kind-hearted but socially awkward freshman at the college.
James Widdoes portrays Robert Hoover, a sensible member of the Delta fraternity who often tries to keep the peace.
Bruce McGill plays Daniel Simpson Day, a laid-back fraternity member known for his easygoing attitude.
Mark Metcalf acts as Doug Neidermeyer, a strict and often harsh member of the rival fraternity.
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