John Cleese portrays Rollo Lee, the ambitious but misguided marketing executive at the center of the zoo's transformation.
Jamie Lee Curtis plays Willa Weston, the no-nonsense executive brought in to shake up the failing zoo and clash with Rollo Lee.
Kevin Kline shines in the dual role of Vince McCain and Rod McCain, two eccentric and contrasting billionaire siblings with plans for the zoo.
Michael Palin embodies Adrian 'Bugsy' Malone, the lovable and somewhat bumbling zookeeper caught in the middle of the corporate chaos.
Robert Lindsay appears as Sydney Lotterby, a smooth-talking TV presenter with his own agenda when it comes to the zoo's future.
Ronnie Corbett takes on the role of Reggie Sealions, a small but pivotal character involved in the zoo's animal antics.
Carey Lowell brings charm to the character of Cub Felines, a zoo employee entangled in both the animal and human drama.
Bille Brown portrays Neville Coltrane, a key figure in the zoo's management who must navigate the chaos caused by Rollo and Willa.
Derek Griffiths plays Gerry Ungulates, adding humor and energy to the zoo's eclectic mix of staff and visitors.
Cynthia Cleese takes on the role of Pip 'Small Mammals', a quirky character integral to the zoo's animal exhibits and behind-the-scenes drama.
Richard Ridings embodies Hugh Primates, further expanding the zoo's colorful array of employees and animals in comedic fashion.
Maria Aitken portrays Di Admin, the efficient and somewhat overlooked administrative figure keeping the zoo's operations running smoothly.
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