Sally Hawkins portrays Elisa Esposito, a mute janitor who forms a unique bond with the Amphibian Man.
Michael Shannon plays Richard Strickland, a cruel and abusive government agent who is in charge of the facility where the Amphibian Man is kept.
Richard Jenkins portrays Giles, a closeted gay man and Elisa's neighbor and close friend who supports her throughout the film.
Octavia Spencer plays Zelda Fuller, Elisa's co-worker and close friend who helps her communicate with the Amphibian Man.
Michael Stuhlbarg portrays Dr. Robert Hoffstetler, a scientist who is revealed to be a Russian spy with conflicting loyalties.
Doug Jones brings to life the Amphibian Man, a mysterious and intelligent creature with whom Elisa forms a deep connection.
David Hewlett appears as Fleming, a researcher who works at the facility and becomes involved in the unfolding events.
Nick Searcy plays General Hoyt, a military official who oversees the operations at the facility where the Amphibian Man is held.
Stewart Arnott portrays Bernard, a scientist working at the facility where the Amphibian Man is being studied.
Nigel Bennett plays Mihalkov, a Soviet operative who is involved in the mission surrounding the Amphibian Man.
Lauren Lee Smith appears as Elaine Strickland, the wife of Richard Strickland who is unhappy with her marriage.
Martin Roach portrays Brewster Fuller, Zelda's husband who also works at the facility alongside Elisa and Zelda.
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