Chevy Chase plays Dusty Bottoms, one of the Three Amigos, a naive and somewhat clueless actor in the trio.
Steve Martin stars as Lucky Day, another member of the Three Amigos known for his quick wit and charm.
Martin Short portrays Ned Nederlander, the third member of the Three Amigos, bringing his comedic talents to the role.
Alfonso Arau plays the menacing antagonist El Guapo who the Three Amigos must confront.
Tony Plana takes on the role of Jefe, El Guapo's right-hand man and enforcer.
Patrice Martinez portrays Carmen, a beautiful damsel in distress who seeks the help of the Three Amigos.
Jorge Cervera Jr. appears as Bandito #1, one of the outlaws encountered by the Three Amigos.
Kai Wulff plays the character German, a member of El Guapo's gang who causes trouble for the heroes.
Abel Franco stars as Papa Sanchez, a father figure in the village where the Three Amigos find themselves.
Fred Asparagus appears as the Bartender, a minor character who interacts with the main cast members.
Jon Lovitz makes a cameo as Morty, a Hollywood agent who represents the Three Amigos.
Joe Mantegna portrays Harry Flugleman, a movie studio executive involved in the Three Amigos' misadventures.
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