Jim Belushi plays Mike Dooley, a hard-nosed police detective who reluctantly partners with a highly intelligent German Shepherd named Jerry Lee to solve a case.
Mel Harris portrays Tracy, the love interest of Mike Dooley who works as a veterinarian and eventually becomes involved in the investigation alongside him.
Kevin Tighe appears as Lyman, a fellow police officer who is skeptical of Mike Dooley's unorthodox methods but eventually comes to respect his dedication to the case.
Ed O'Neill plays the role of Brannigan, a gruff police captain who initially clashes with Mike Dooley but gradually warms up to his unconventional tactics.
James Handy is Captain Byers, a higher-ranking officer who oversees the police department and serves as a mentor figure to the team working on the case.
Daniel Davis portrays Halstead, a sophisticated and meticulous detective who provides valuable insight and assistance to Mike Dooley throughout the investigation.
Cotter Smith plays Gilliam, a seasoned detective who teams up with Mike Dooley and Jerry Lee to track down the perpetrators behind a series of crimes.
John Snyder appears as Freddie, a criminal involved in the case who finds himself outmatched by the unconventional tactics of Mike Dooley and his canine partner.
Pruitt Taylor Vince voices Benny, the highly intelligent and loyal German Shepherd named Jerry Lee who becomes Mike Dooley's trusted companion and crime-solving partner.
Sherman Howard portrays Dillon, a menacing criminal mastermind who poses a serious threat to both the police force and the safety of the community.
Jeff Allin plays Chad, a member of the police team who assists Mike Dooley and his colleagues in unraveling the complex web of criminal activities.
David Haskell appears as Doctor, a medical professional who provides crucial assistance to the police in determining the cause of certain crimes and aiding in the investigation.
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