Harley Quinn (TV) Cast
The cast of Harley Quinn. Updated on October 3, 2025.
97 actors
Kaley Cuoco
as Harley Quinn (voice) (47 episodes)

Kaley Cuoco brings Harley Quinn, the show's main character, to life with her voice talents. Cuoco's portrayal captures Harley's complexity, wit, and unpredictable nature. She immerses herself in the role, delivering a standout performance.

Lake Bell
as Poison Ivy (voice) (47 episodes)

Lake Bell voices the character of Poison Ivy, a skilled botanist with a strong sense of environmental justice. Bell's portrayal highlights Poison Ivy's intelligence and her complex relationship with Harley Quinn.

Giancarlo Esposito
as Lex Luthor (voice) (11 episodes)

Giancarlo Esposito lends his voice to Lex Luthor, a mastermind and ruthless businessman. Esposito's portrayal brings out the charm, cunning, and calculated nature of this iconic Batman villain.

Sean Giambrone
as Joshua (voice) (3 episodes)

Sean Giambrone voices Joshua, a young and enthusiastic individual who gets caught up in Harley Quinn's adventures. Giambrone's voice acting brings out Joshua's innocence, curiosity, and loyalty.

Wayne Knight
as The Penguin (voice) (2 episodes)

Wayne Knight injects humor and personality into the role of The Penguin, a notorious villain in Gotham City. Knight's voice brings out The Penguin's cunning, ambition, and occasional bumbling nature.

Ron Funches
as King Shark (voice) (31 episodes)

Ron Funches lends his voice to the character of King Shark, a fearsome yet hilarious half-human, half-shark creature. Funches' voice acting captures King Shark's vocal uniqueness, tough exterior, and gentle soul.

Howie Mandel
as Howie Mandel (voice) (1 episode)

Howie Mandel voices a minor character named Howie Mandel in the show. Mandel's distinct voice brings comedic flair to the character, providing entertaining moments throughout the series.

George López
as George Lopez (voice) (1 episode)

George Lopez adds his vocal talents to the character of George Lopez in the show. Lopez's voice portrayal adds a distinctive and enjoyable element to the series, enhancing the overall comedic tone.

Billy Bob Thornton
as Billy Bob Thornton (voice) (1 episode)
James Gunn
as James Gunn (voice) (2 episodes)
Cathy Ang
as Golden Glider (voice) (1 episode)
Alfred Molina
as Mr. Freeze / Stew (voice) (1 episode)
Sanaa Lathan
as Catwoman (voice) (9 episodes)
Larry Owens
as Music Meister (voice) (1 episode)
Sam Richardson
as Swamp Thing (voice) (2 episodes)
Matt Ryan
as Constantine (voice) (1 episode)
Krizia Bajos
as Bethany (voice) (3 episodes)
James Corleto
as Benicio (voice) (2 episodes)
Amy Sedaris
as Debbie (voice) (1 episode)
Larissa Gallager
as Prawn (voice) (1 episode)
Griffin Newman
as Mad Hatter (voice) (1 episode)
Jack Stanton
as Little Bruce (voice) (1 episode)
Rory Scovel
as Gus / Assassin (voice) (1 episode)
Matt Oberg
as Kiteman (voice) (10 episodes)
Tisha Campbell
as Tawny Young (voice) (6 episodes)
Michael Ironside
as Darkseid (voice) (4 episodes)
Jessica Walter
as Wendy / Granny Goodness (voice) (1 episode)
Briana Cuoco
as Batgirl (voice) (9 episodes)
Jim Rash
as The Riddler (voice) (5 episodes)
Jameela Jamil
as Eris (voice) (1 episode)
Jacob Tremblay
as Robin (voice) (6 episodes)
Justina Machado
as Bethany (voice) (1 episode)
James Adomian
as Bane (voice) (10 episodes)
Tony Hale
as Dr. Psycho (voice) (17 episodes)
Alan Tudyk
as Clayface / The Joker (voice) (13 episodes)
Scott Porter
as The Flash (voice) (1 episode)
Andy Daly
as Two-Face (voice) (5 episodes)
Jonah Platt
as Singing Clayface (voice) (1 episode)
Will Sasso
as Maxie Zeus (voice) (1 episode)
Rachel Dratch
as Nora Freeze (voice) (8 episodes)
Meryl Hathaway
as Marcus (voice) (1 episode)
Rahul Kohli
as Scarecrow (voice) (5 episodes)
Nicole Sullivan
as Mrs. Cobblepot / Benjamin (voice) (1 episode)
Phil LaMarr
as Black Manta / Tentacle Monster (voice) (1 episode)
Diedrich Bader
as Bruce Wayne (voice) (2 episodes)
James Wolk
as Superman (voice) (6 episodes)
Tom Hollander
as Alfred (voice) (7 episodes)
Wanda Sykes
as Queen of Fables (voice) (3 episodes)
Harvey Guillén
as Nightwing (voice) (5 episodes)
Natalie Morales
as Lois Lane (voice) (2 episodes)
Frankie Muniz
as Frankie Muniz (voice) (1 episode)
Suzy Nakamura
as Realtor (voice) (1 episode)
Rhea Perlman
as Golda (voice) (1 episode)
Tom Kenny
as The Arm (voice) (1 episode)
Mark Whitten
as The Cowled Critic / Herman (voice) (1 episode)
Brad Morris
as Businessman / Prisoner (voice) (1 episode)
Charlie Adler
as Nick / Grandpa (voice) (1 episode)
Susie Essman
as Sharon / Grandma (voice) (1 episode)
Vanessa Marshall
as Wonder Woman (voice) (2 episodes)
Mary Holland
as Jennifer / Tabitha (voice) (2 episodes)
Chris Diamantopoulos
as Aquaman / Chef (voice) (1 episode)
Christopher Meloni
as Commissioner Gordon (voice) (13 episodes)
Kari Wahlgren
as Plastique / Queen Elizabeth II (voice) (1 episode)
Gary Anthony Williams
as Brother Prince Shark (voice) (1 episode)
JB Smoove
as Frank the Plant (voice) (21 episodes)
Leila Birch
as Enchantress / Lover #1 (voice) (1 episode)
Quinta Brunson
as Hawkgirl (voice) (1 episode)
Brett Goldstein
as Brett Goldstein (voice) (1 episode)
Josh Helman
as Captain Boomerang (voice) (1 episode)
John Stamos
as Etrigan the Demon (voice) (1 episode)
Janet Varney
as Mera (voice) (1 episode)
Tyler James Williams
as Hawkman (voice) (1 episode)
Casey Wilson
as Betty (voice) (1 episode)
Talia Tabin
as Mrs. Calendar (voice) (1 episode)
Keith Ferguson
as Jonas / Steppenwolf / Groundskeeper (voice) (1 episode)
Ben Levin
as Captain Cold (voice) (5 episodes)
Sammi Corona-Lampa
as Sofia (voice) (2 episodes)
Leilani Barrett
as Pool Boy #2 (voice) (1 episode)
Eric Bauza
as Starro / Bouncing Boy (voice) (1 episode)
Amy K. Harmon
as Ramona / Drunk Woman (voice) (1 episode)
Justin Willman
as Magician / Buffet Man / Deejay (voice) (1 episode)
Jeannie Tirado
as Volcana (voice) (4 episodes)
Larissa Gallagher
as Recepitonist / Italian Boy (voice) (1 episode)
Lacey Chabert
as Supergirl (voice) (1 episode)
Paul W. Downs
as The Jons (voice) (2 episodes)
Aline Elasmar
as Talia Al Ghul / Livewire (voice) (3 episodes)
Edi Patterson
as Veronica Cale (voice) (3 episodes)
Zeno Robinson
as The Flash (voice) (1 episode)
Rain Valdez
as Alysia (voice) (1 episode)
Kerry Knuppe
as Terra (voice) (2 episodes)
Kimberly Brooks
as Little Girl #1 (voice) (1 episode)
Vico Ortiz
as Tefe (voice) (2 episodes)
Amari McCoy
as Mitzi (voice) (1 episode)
Zosia Mamet
as Princess Ladyfingers (voice) (1 episode)
Janeane Garofalo
as Signora/Mama (voice) (1 episode)
Eric Morgan Stuart
as Scientist (voice) (1 episode)
Gregg Turkington
as Clegg (voice) (1 episode)
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